Cryopreservation and Resuscitation
????? ??? ?? ??? ??? ????? ???? ?? ??? ?? ??? ?? ??? ?? ?? ??? ???????. ??? ?? ?? ??? ???? ? ??? ??? ??? ???? ??? ???? ???? ?? ??? ??? ?? ???? ?? ?? ? ????. ?? ??? ?? ??? ????, ??? ??? ??? ???? ??? ? ????(?? ?? ??? ??). ??? ?? ????? ???? ? ?? ??? ???? ?? ????? ????, ?? ??? ??? ??? ?? ?? ??? ???? ???? SPF ??? ???? ??? ? ??? ???.
??
????? ??? ???? ????, ?? ?? ? ???? 70% ??, ?? ?? ? ???? 95% ?????.
???? ??? ??? ??? ???? ????, ?? ???? ??? ?? ??? ????. ?? ??? ??? ?? ?? ?? ? ?? ?? ???? ??? ???, ??? ? ??? ?? ???? ???? ??
?? ?? ??? ???? ?? ?? ?? ???? ???? ??? ???? ???
Cryopreservation Method | Sperm cryopreservation & resuscitation | Embryo cryopreservation & resuscitation |
Quantity of mice used | 3 fertile adult males | 3 fertile adult males and 30-40 females |
Amount of genetic material | Cryopreservation of more than 20 vials of sperm | Cryopreservation of approximately 400 embryos with 30-40 embryos per vial |
Technical features | After sperm resuscitation, IVF allows for the rapid production of large number of offspring of the same DOB. | Embryos can be transferred directly after resuscitation without the need for an IVF procedure. |
Suitable for heterozygous or homozygous breeding strains. | Suitable for breeding single or multiple gene homozygous or pure inbred background strains. | |
Experience | Nearly 5,000 strains of sperm cryopreserved | Nearly 1,400 strains of embryo cryopreserved |
